Associate Scientist, Translational Pharmacology

All qualified applicants will receive consideration for employment without regard to race, color, sex, sexual orientation, gender identity, religion, national origin, disability, veteran status, age, marital status, pregnancy, genetic information, or other legally protected status.

Description:

Works independently to design, perform, interpret, and record in vitro cellular functional assays with lead candidate antibodies to achieve program goals within the translational pharmacology group and project team. Responsibilities include developing and executing cell-based functional assays with cell lines, primary cells, whole blood, PBMCs and sub-populations of immune cells from both normal and disease donors to understand the biology of targets, evaluate the pharmacology of lead antibody candidates, support translational studies, and analyze in vivo pharmacology (PK/PD) relationships. Additional responsibilities include evaluating activity of antibodies from AnaptysBio's discovery platforms to support antibody drug screening, target validation, mechanism-of-action studies, biomarker identification, and/or companion diagnostics development. Requires deep knowledge and application of both theoretical and technical aspects of experimental methods from relevant scientific literature. Requires the ability to think critically, understand data analysis and presentation, and communicate experimental findings in both individual and team settings.

Essential Functions

• Isolate and purify primary cells from multiple sources (blood, spleen, skin) and species (human, NHP, mouse)
• Perform mammalian tissue culture, including both established cell lines and primary immune cells
• Develop and optimize in vitro functional assays for T, B cells, myeloid cells, including both basic and more complex co-culture systems, such as cell proliferation, cell signaling, and cytokine production
• Perform high dimensional flow cytometry (both surface and intracellular markers) and cytokine analysis using ELISA, MSD, and/or Luminex platforms
• Analyze complex scientific datasets using GraphPad PRISM to interpret translational pharmacology
• Collaborate effectively with team members in other departments (Molecular Biology, Protein Sciences, Mechanism of Action, In vivo) to achieve program goals
• Present experimental results and make recommendations for action/further research at project and department meetings
• Prepare research reports for transfer to external CRO/collaborators, patent applications, and posters/manuscripts
• Formulate experimental plans to achieve scientific goals in new and creative ways

Requirements:

• Bachelor's degree in a relevant scientific discipline (biology, immunology, translational pharmacology, or related field) with 10 years related laboratory experience or equivalent

Knowledge and Competencies:

• Deep knowledge and working experience in principles and techniques of cellular immunology, cell biology, and translational pharmacology (cell culture, in vitro cell-based functional assays, flow cytometry, analyte platforms, familiarity with animal models of autoimmune disease and PK/PD principles)
• Experience in industry drug discovery workflow, from linking antibody screening to in vitro/ in vivo translational assays that support program advancement and IND filing
• Antibody discovery experience in screening assays
• Scientific independence, ability to work with limited supervision, ability to collaborate effectively within team setting and cross-functionally with other departments
• Ability to quickly implement standard approaches and ensure experiments are well-designed and performed to a high standard
• Ability to develop, apply, and troubleshoot new methods to address specific scientific questions
• Proficiency in data analysis and presentation applications (Flowjo, Diva, Novoexpress, etc.; ELN/Benchling, Word, Excel, Powerpoint)

Desired Experience

• Ex vivo human or animal explant systems to evaluate tissue pharmacology (for example involving TEER, cell migration, organoids, fibrosis).
• Quantitative pharmacology of target and therapeutic antibody trafficking using live-cell imaging, high-content imaging, and immune synapse formation assays
• Development of PK/ADA assays for therapeutic antibodies
• Molecular immunology to support translational medicine approaches (scRNAseq, spatial transcriptomics, multiplex IHC)
• Development of new methods for antibody discovery leveraging novel molecular and/or cell biology
• Management/mentorship of more junior scientists

Physical Demands

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

While performing the duties of this job, the employee is regularly required to sit and talk or hear. The employee frequently is required to use hands to finger, handle, or feel. The employee is occasionally required to stand, walk, and reach with hands and arms. The employee must occasionally lift and/or move up to 10 pounds. Specific vision abilities required by this job include close vision and ability to adjust focus in order to read.

Work Environment

The work environment characteristics are representative of those an employee encounters while performing the essential functions of this job, typically in an open office environment. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

The noise level in the work environment is usually moderate.

May be required to travel by plane or car.

This position requires working with biological and/or chemical hazards.

All qualified applicants will receive consideration for employment without regard to race, color, sex, sexual orientation, gender identity, religion, national origin, disability, veteran status, age, marital status, pregnancy, genetic information, or other legally protected status

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